Product Details
Synonyms
GZMB, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT, Fragmentin-2, Granzyme B, Granzyme-2, GRB
Source
Human Granzyme B Protein, Tag Free (GZB-H5213) is expressed from human 293 cells (HEK293). It contains AA Ile 21 - Tyr 247 (Accession # P10144).
Predicted N-terminus: Ile 21
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 25.5 kDa. The protein migrates as 34-37 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Performance Data
SDS-PAGE

Human Granzyme B Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity
Measured by its ability to cleave the fluorogenic peptide substrate, Ac-Ile-Glu-Thr-Asp-AFC. The specific activity is >140 pmol/min/μg (QC tested).
Protocol
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