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PE-Labeled Human GUCY2C / Guanylyl cyclase C Protein, His Tag (Site-specific conjugation)

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Numéro de produit/spécification
Prix
Qté
GUC-HP2E3-25tests
$380.00
GUC-HP2E3-200tests
$2450.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    GUCY2C, GUC2C, STAR, STA receptor, hSTAR, GC-C

  • Source

    PE-Labeled Human GUCY2C, His Tag (GUC-HP2E3) is produced via site-specific conjugation of PE to Human GUCY2C, His Tag under optimal conditions with a proprietary technology. Human GUCY2C, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gln 430 (Accession # P25092-1).

    Predicted N-terminus: Ser 24

    Request for sequence

  • Molecular Characterization

    GUCY2C Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 49.6 kDa.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Application

    Please note that this product is NOT compatible to streptavidin detection system.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • Bioactivity-FACS

     GUCY2C FACS

    5e5 of anti-GUCY2C CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human GUCY2C, His Tag (Cat. No. GUC-HP2E3) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

    Protocol

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Introduction de fond

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Heat-stable enterotoxin receptor

  • Catégorie:

  • Nombre de médicaments répertoriés:

    1 Détails

  • Nombre de médicaments cliniques:

    10 Détails

  • Étape de développement la plus élevée:

    Approved

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