APC-Labeled Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer Protein

Synonyms

HLA-A*0201 & B2M & MAGE-A4 (GVYDGREHTV)

Source

APC-Labeled Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer Protein (HL4-HA2E3) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & GVYDGREHTV peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & GVYDGREHTV).

Predicted N-terminus: Gly 25 & Ile 21

Molecular Characterization

APC-Labeled Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer Protein is assembled by biotinylated monomer and APC-labeled streptavidin.

Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein is produced by co-expression of HLA-A*02:01 and B2M loaded with MAGE-A4 peptide. Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.3 kDa and 11.7 kDa.

Conjugate

APC

Excitation Wavelength: 640 nm

Emission Wavelength: 661 nm

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, 50-150 mM NaCl with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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