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HLA-A*1101 & B2M & WT1 (FSCRWPSCQK)
Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer Protein (HLW-HA2H4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & FSCRWPSCQK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & FSCRWPSCQK).
Predicted N-terminus: Gly 25 & Ile 21
This protein carries a polyhistidine tag at the C-terminus.
Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer Protein is assembled by biotinylated monomer and AF647-labeled streptavidin.
Biotinylated Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Complex Protein is produced by co-expression of HLA and B2M loaded with WT-1 peptide. Biotinylated Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
AF647
Excitation Wavelength: 640 nm
Emission Wavelength: 672 nm
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF647 using standard chemical labeling method. The residual AF647 is removed by molecular sieve treatment during purification process.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.