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Rabbit IgG Fc Protein, Tag Free (MALS verified)

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Numéro de produit/spécification
Prix
Qté
IGG-R5203-200ug
$280.00
IGG-R5203-1mg (500ug X 2)
$740.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    IgG Fc

  • Source

    Rabbit IgG Fc, Tag Free (IGG-R5203) is expressed from human 293 cells (HEK293). It contains AA Cys 105 - Lys 323 (Accession # P01870-1).

    Predicted N-terminus: Cys 105

    Request for sequence
  • Molecular Characterization

    IgG Fc Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries no "tag". The protein has a calculated MW of 24.8 kDa. The protein migrates as 28-32 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    IgG Fc SDS-PAGE

    Rabbit IgG Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IgG Fc SEC-MALS

    The purity of Rabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) is more than 95% and the molecular weight of this protein is around 52-66 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IgG Fc ELISA

    Immobilized Rabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.3-5 ng/mL (QC tested).

    Protocol
  •  IgG Fc ELISA

    Immobilized Rabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) with a linear range of 0.02-0.625 μg/mL (Routinely tested).

    Protocol

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Introduction de fond

Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport).

Progrès de frontière

 
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  • Informations de base sur le produit
  • Détails du produit
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  • Affichage des données
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