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Détails du produit
Synonyms
IgG1
Source
Human IgG1 Fc Protein, His Tag-Cy5 (IG1-HC2H3) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 330 (Accession # P01857-1 (C103S, M135Y, S137T, T139E, H316K, N317F)).
Predicted N-terminus: Glu 99
Request for sequenceMolecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.1 kDa.
Conjugate
Cy5
Excitation Wavelength: 651 nm
Emission Wavelength: 670 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with Cy5 using standard chemical labeling method. The residual Cy5 is removed by molecular sieve treatment during purification process.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
Bioactivity-SPR
Human IgG1 Fc Protein, His Tag-Cy5 (IG1-HC2H3) immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) with an affinity constant of 8.27 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-Flow Cytometry
Flow cytometric analysis of HEK293/Human FcRn (FCGRT & B2M) Stable Cell staining with Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F) Protein, His Tag-Cy5 (Cat. No. IG1-HC2H3) at 1:50 dilution(2 μL of the stock solution corresponds to labeling of 2e5 cells in a final volume of 100 µL) , compared with negative control protein. Cy5 signal was used to evaluate the binding activity (QC tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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