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Mouse IgG3 Fc Protein, Tag Free (MALS verified)

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Numéro de produit/spécification
Prix
Qté
IG3-M5214-200ug
$325.00
IG3-M5214-1mg (500ug X 2)
$745.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    Ig gamma-3 chain C region, IgG, IgG3

  • Source

    Mouse IgG3 Fc Protein, Tag Free (IG3-M5214) is expressed from human 293 cells (HEK293). It contains AA Pro 98 - Pro 327 (Accession # P03987-2).

    Request for sequence
  • Molecular Characterization

    IgG3 Fc Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries no "tag".

    The protein has a calculated MW of 26.1 kDa. The protein migrates as 30-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    IgG3 Fc SDS-PAGE

    Mouse IgG3 Fc Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IgG3 Fc SEC-MALS

    The purity of Mouse IgG3 Fc Protein, Tag Free (Cat. No. IG3-M5214) is more than 95% and the molecular weight of this protein is around 60-70 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IgG3 Fc ELISA

    Immobilized Mouse IgG3 Fc Protein, Tag Free (Cat. No. IG3-M5214) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.6-10 ng/mL (QC tested).

    Protocol

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Introduction de fond

Immunoglobulin G3 (IgG3) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc / IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3).

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Immunoglobulin G

  • Catégorie:

  • Nombre de médicaments répertoriés:

    1 Détails

  • Nombre de médicaments cliniques:

    7 Détails

  • Étape de développement la plus élevée:

    Approved

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