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Détails du produit
Synonyms
IL-12 R beta 1, IL12RB1, CD212, IL12R, IL12RB, IL-12RB1
Source
Biotinylated Cynomolgus IL-12 R beta 1 , His,Avitag (ILB-C52E2) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Asp 545 (Accession # G7PWY9-1).
Predicted N-terminus: Cys 24
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 61.0 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Biotinylated Cynomolgus IL-12 R beta 1 , His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Biotinylated Cynomolgus IL-12 R beta 1 , His,Avitag (Cat. No. ILB-C52E2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-12B, Fc Tag (Cat. No. NK2-H5258) with a linear range of 0.1-8 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Cynomolgus IL-12 R beta 1 , His,Avitag (Cat. No. ILB-C52E2) on SA Biosensor, can bind ActiveMax® Human IL-12 ,Tag Free (Cat. No. IL2-H5210) with an affinity constant of 1.9 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Interleukin-12 receptor
- Catégorie：
- Nombre de médicaments répertoriés：
0 Détails
- Nombre de médicaments cliniques：
9 Détails
- Étape de développement la plus élevée：
Phase 2 Clinical
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