Biotinylated Human IL-20 Protein, His,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
IL0-H82E3-25ug
$360.00
IL0-H82E3-200ug
$1565.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    interleukin-20, IL10D, IL-20, interleukin 20, Cytokine Zcyto10, ZCYTO10

  • Source

    Biotinylated Human IL-20 Protein, His,Avitag (IL0-H82E3) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Glu 176 (Accession # Q9NYY1-1).

    Predicted N-terminus: Leu 25

    Request for sequence
  • Molecular Characterization

    IL-20 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 21.1 kDa. The protein migrates as 21-23 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-20 SDS-PAGE

    Biotinylated Human IL-20 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL-20 SEC-MALS

    The purity of Biotinylated Human IL-20 Protein, His,Avitag (Cat. No. IL0-H82E3) is more than 90% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IL-20 ELISA

    Immobilized Biotinylated Human IL-20 Protein, His,Avitag (Cat. No. IL0-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-20 R alpha, Fc Tag (Cat. No. ILA-H525d) with a linear range of 10-78 ng/mL (QC tested).

    Protocol

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Background

Interleukin-20 (IL-20) belongs to the family of IL-10-related cytokines, which includes IL-10, -19, -20, -22, -24 and -26. IL-20 induces STAT activation through two types of receptor complex, one of which it shares with IL-19, indicating that their biological activities may overlap. Both cytokines may play an important role in regulating the development and proper functioning of skin. IL-20 has also been shown to promote endothelial cell tube formation, suggesting that it may be involved in vascular vessel maturation and remodelling.

Recent Advances

 
Drug Development Progress
  • English Name:

    Interleukin 20

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    0 Details

  • Highest Development Stage:

    Preclinical

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