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Biotinylated Human IL-22 Protein, His,Avitag™

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Numéro de produit/spécification
Prix
Qté
IL2-H8247-25ug
$360.00
IL2-H8247-200ug
$1565.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    IL-22, IL-TIF, ZCYTO18

  • Source

    Biotinylated Human IL-22, His,Avitag (IL2-H8247) is expressed from human 293 cells (HEK293). It contains AA Ala 34 - Ile 179 (Accession # Q9GZX6-1).

    Predicted N-terminus: His

    Request for sequence
  • Molecular Characterization

    IL-22 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 20.3 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    IL-22 SDS-PAGE

    Biotinylated Human IL-22, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     IL-22 ELISA

    Immobilized Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) at 1 μg/mL (100 μL/well)on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate. can bind Human IL-22 Antibody with a linear range of 0.1-4 ng/mL (QC tested).

    Protocol
  •  IL-22 ELISA

    Immobilized Human IL-22 Antibody at 1 μg/mL (100 μL/well) can bind Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) with a linear range of 1-31 ng/mL (Routinely tested).

    Protocol
  •  IL-22 ELISA

    Immobilized Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-22 R alpha 1, Fc Tag with a linear range of 0.6-78 ng/mL (Routinely tested).

    Protocol
  •  IL-22 ELISA

    Immobilized Human IL-22BP Protein, Fc Tag (Cat. No. ILP-H5254) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) with a linear range of 0.03-1 ng/mL (Routinely tested).

    Protocol
  • Bioactivity-SPR

     IL-22 SPR

    Biotinylated Human IL-22, His,Avitag (Cat. No. IL2-H8247) captured on Biotin CAP-Series S Sensor Chip can bind Human IL-22BP Protein, His Tag (Cat. No. ILP-H52H3) with an affinity constant of 0.162 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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  • V***s2025-07-19
    J'aime
    We purchased the "Biotinylated Human IL-22 Protein, His,Avitag™" from ACRO Biosystems. The quality of this product is great and behaves as expected in our experiments. The product support is great and you can find all the information you need on the website. The only suggestion I would have for this is to make it easier and faster to get protocols and data sheets without having to enter all you basic information to be able to download them. Other than that the only other "negative" review I would have is that there are hardly any discounts or promotions for this product. We bought many of the 25ug vials and, in hindsight, we should have ordered the 200ug to be a little more cost effective. Other than that, communication with a representative is fast and easy if needed.

Introduction de fond

Interleukin-22 (IL22) is also known as cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor (IL-TIF), which is belongs to the IL-10 family or IL-10 superfamily (including IL-19, IL-20, IL-24, and IL-26), a class of potent mediators of cellular inflammatory responses. IL-22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein IL-22BP. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Interleukin-22

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    3 Détails

  • Étape de développement la plus élevée:

    Phase 3 Clinical

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