Human IL-33 (2-270) Protein, His Tag

Détails du produit

Synonyms
IL33, DV27, C9ORF26, IL1F11, NFHEV, DKFZp586H0523, DVS27, NFEHEV, RP11-575C20.2
Source
Human IL-33 (2-270) Protein, His Tag (IL3-H51H3) is expressed from E. coli cells. It contains AA Lys 2 - Thr 270 (Accession # O95760-1).
Predicted N-terminus: Met
Request for sequence
Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 32.6 kDa. The protein migrates as 8 kDa,29 kDa and 33-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. The product MUST be stored at -70°C or lower upon receipt;
2. -70°C for 12 months under sterile conditions.
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SDS-PAGE
Human IL-33 (2-270) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
Immobilized Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) at 5 μg/mL (100 μL/well) can bind Human IL-33 (2-270) Protein, His Tag (Cat. No. IL3-H51H3) with a linear range of 0.01-0.625 μg/mL (QC tested).
Protocol

Bioactivity-BLI
Loaded Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) on Protein A Biosensor, can bind Human IL-33 (2-270) Protein, His Tag (Cat. No. IL3-H51H3) with an affinity constant of 15.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol

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Introduction de fond

Interleukin 33 (IL33) is known as C9orf26, DKFZp586H0523, DVS27, NF-HEV, NFEHEV, RP11-575C20.2,and is a cytokine belonging to the IL-1 superfamily. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL-33 mediates its biological effects by interacting with the receptors ST2 (aka IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs.

Progrès de frontière

Progrès du développement médicamenteux

Nom anglais：
Interleukin-33
Catégorie：
Nombre de médicaments répertoriés：
0 Détails
Nombre de médicaments cliniques：
9 Détails
Étape de développement la plus élevée：
Phase 3 Clinical

Drug Candidate Licensing

Human IL-33 (2-270) Protein, His Tag

Détails du produit

Synonyms

Source

Molecular Characterization

Endotoxin

Purity

Formulation

Shipping and Storage

ACRO Quality Management System

Les clients ont également consulté

Affichage des données

SDS-PAGE

Bioactivity-ELISA

Bioactivity-BLI

Avis des utilisateurs Publier un commentaire

Introduction de fond

Progrès de frontière

Avis des utilisateurs
Publier un commentaire