Human IL-36Ra Protein, Tag Free (HPLC & SPR verified)

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Cat. No. / Size
Price
Qty
ILA-H5117-100ug
$310.00
ILA-H5117-1mg (500ug X 2)
$2245.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    IL-36Ra, FIL1 delta, IL-1RP3, IL-1HY1, IL-1 delta, IL-1F5, IL-1L1, IL1F5, IL36Ra, IL36RN

  • Source

    Human IL-36Ra Protein, Tag Free (ILA-H5117) is expressed from E. coli cells. It contains AA Val 2 - Asp 155 (Accession # Q9UBH0).

    Predicted N-terminus: Val

    Request for sequence
  • Molecular Characterization

    IL-36Ra Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries no "tag".

    The protein has a calculated MW of 16.8 kDa. The protein migrates as 15-16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-36Ra SDS-PAGE

    Human IL-36Ra Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-HPLC

    IL-36Ra SEC-HPLC

    The purity of Human IL-36Ra Protein, Tag Free (Cat. No. ILA-H5117) was greater than 90% as determined by SEC-HPLC.

  • Bioactivity-SPR

     IL-36Ra SPR

    Biotinylated Human IL-1 Rrp2, His,Avitag (Cat. No. IL2-H82E9) immobilized on SA Chip can bind Human IL-36Ra Protein, Tag Free (Cat. No. ILA-H5117) with an affinity constant of 2.32 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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Background

The IL-36 subfamily of cytokines is the most diverse, including three agonists(IL-36α, IL-36β, and IL-36γ), one antagonist(IL-36Ra), one cognate receptor(IL-36R) and one accessory protein(IL-1RAcP). The receptor activation follows a two-step mechanism in that the agonist first binds to IL-36R and the resulting binary complex recruits IL-1RAcP. IL-36Ra inhibits the activity of IL-36 (IL-36α, IL-36β, and IL-36γ) by binding to the receptor IL-36R and preventing IL-36R from associating with the coreceptor IL-1RAcP for signaling.

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