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Product Details
Synonym
IL4R, CD124, IL4RA
Source
Human IL-4 R alpha, Fc Tag (ILR-H5253) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 (Accession # P24394-1).
Predicted N-terminus: Met 26
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 50.4 kDa. The protein migrates as 60-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human IL-4 R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) is more than 95% and the molecular weight of this protein is around 113-138 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human IL-4, premium grade (Cat. No. IL4-H4218) at 5 μg/mL (100 μL/well)can bind Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) with a linear range of 1-20 ng/mL (QC tested).
Protocol
Immobilized Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) at 5 μg/mL (100 μL/well)can bind Biotinylated Human IL-4, Avitag,His Tag (Cat. No. IL4-H82E0) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Serial dilutions of Monoclonal IL-4R/CD124 Neutralizing Antibody were added into Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253): Biotinylated Human IL-4, Avitag,His Tag (Cat. No. IL4-H82E0) binding reactions. The half maximal inhibitory concentration (IC50) is 1.144 μg/mL (Routinely tested).
Protocol
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Background
Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL13Ra1 to form the type II receptor which is responsive to both IL4 and IL13. Interleukin-4 receptor has been shown to interact with SHC1.
Recent Advances
Drug Development Progress- English Name：
Interleukin-4 receptor subunit alpha
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
19 Details
- Highest Development Stage：
Approved
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