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Product Details
Synonyms
Integrin alpha 4 beta 7, ITGA4&ITGB7
Source
Biotinylated Human ITGA4&ITGB7 Heterodimer Protein, His,Avitag&Tag Free (IT7-H82W4) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Glu 20 - His 723 (ITGB7) (Accession # P13612-1 (ITGA4) & P26010-1 (ITGB7)).
Predicted N-terminus: Tyr 34 (ITGA4) & Glu 20 (ITGB7)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Biotinylated Human ITGA4&ITGB7 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGA4 and ITGB7, has a calculated MW of 113.1 kDa (ITGA4) & 81.8 kDa (ITGB7). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB7 contains no tag but a basic tail at the C-terminus. The protein migrates as 65-80 kDa, 95-130 kDa and 140-160 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human ITGA4&ITGB7 Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human ITGA4&ITGB7 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT7-H82W4) is more than 90% and the molecular weight of this protein is around 200-240 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human ITGA4&ITGB7 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT7-H82W4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-Human Integrin α 4 β 7 MAb, Human IgG1(ADCC Disable) with a linear range of 0.02-1 ng/mL (QC tested).
Protocol
Immobilized Human MAdCAM-1, Fc Tag (Cat. No. MAM-H5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4&ITGB7 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT7-H82W4) with a linear range of 0.005-0.156 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Integrin alpha-4/beta-7
- Category：
- Approved Drugs：
4 Details
- Drugs in Clinical Trials：
14 Details
- Highest Development Stage：
Approved
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