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Integrin alpha 5 beta 1, ITGA5&ITGB1
Cynomolgus ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-C52W7) is expressed from human 293 cells (HEK293). It contains AA Phe 42 - Tyr 999 (ITGA5) & Gln 161 - Asp 868 (ITGB1) (Accession # XP_005571144.3 (ITGA5) & A0A7N9D0D7 (ITGB1)).
Predicted N-terminus: Phe 42 (ITGA5) & Gln 161 (ITGB1)
Cynomolgus ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 111.2 kDa (ITGA5) & 83.6 kDa (ITGB1). Subunit ITGA5 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 130-160 kDa (ITGA5) and 95-110 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (NR) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Less than 1.0 EU per μg by the LAL method / rFC method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.