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Détails du produit
Synonyms
Integrin alpha 6 beta 4, ITGA6&ITGB4
Source
Human ITGA6&ITGB4 Heterodimer Protein, His Tag&Tag Free (IN4-H52W3) is expressed from human 293 cells (HEK293). It contains AA Phe 24 - Gly 1012 | Asn 28 - Ser 710 (Accession # P23229-2 & P16144-2).
Predicted N-terminus: Phe 24 | Asn 28
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Human ITGA6&ITGB4 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA6 and ITGB4, has a calculated MW of 116.8 kDa (ITGA6) and 81.8 kDa (ITGB4). Subunit ITGA6 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB4 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-140 kDa (ITGA6) and 75-85 kDa (ITGB4) when calibrated against Star Ribbon Pre-stained Protein Marker respectively due to glycosylation.
The protein is designed as a heterodimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 0.2 M Arginine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human ITGA6&ITGB4 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human ITGA6&ITGB4 Heterodimer Protein, His Tag&Tag Free (Cat. No. IN4-H52W3) is more than 90% and the molecular weight of this protein is around 195-220 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human ITGA6&ITGB4 Heterodimer Protein, His Tag&Tag Free (Cat. No. IN4-H52W3) at 5 μg/mL (100 μL/well) can bind Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) with a linear range of 0.8-13 ng/mL (QC tested).
Protocol
Immobilized Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) at 5 μg/mL (100 μL/well) can bind Human ITGA6&ITGB4 Heterodimer Protein, His Tag&Tag Free (Cat. No. IN4-H52W3) with a linear range of 0.01-0.156 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human Laminin 332 Protein, premium grade (Cat. No. LA3-H5216) immobilized on CM5 Chip can bind Human ITGA6&ITGB4 Heterodimer Protein, His Tag&Tag Free (Cat. No. IN4-H52W3) with an affinity constant of 53.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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