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Product Details
Laminin 332 is indispensable for the efficient and specific differentiation of human induced pluripotent stem cells (iPSCs) into corneal epithelial cells. This laminin isoform is a fundamental component of the corneal epithelial basement membrane in vivo, where it provides not just structural anchorage but also essential pro-differentiation signals. When used as a coating substrate in vitro, LN332 mimics this native niche, powerfully directing iPSCs towards the corneal epithelial lineage. It achieves this by engaging with cellular integrins (primarily α6β4), which triggers signaling pathways that suppress alternative cell fates and actively promote the expression of key corneal epithelial markers like KRT3 and KRT12. This makes LN332 a critical tool for generating authentic, functional corneal epithelial cells for applications in disease modeling, drug testing, and the development of regenerative therapies for ocular surface disorders.
Expression System
HEK293
Key Parameter
Purity (SDS PAGE)> 95%Mycoplasma TestNegativeSterility TestNegativeEndotoxin Test<0.01 EU per μgHost Cell Protein<0.5 ng/µgProtein A<5 ppmFormulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
Bioactivity-SPR
Human Laminin 332 Protein, premium grade (Cat. No. LA3-H5216) immobilized on CM5 Chip can bind Human ITGA6&ITGB4 Heterodimer Protein, His Tag&Tag Free (Cat. No. IN4-H52W3) with an affinity constant of 53.1 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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