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Biotinylated Human LILRB2 / CD85d / ILT4 Protein, His,Avitag™ (MALS verified)

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Numéro de produit/spécification
Prix
Qté
LI2-H82E7-25ug
$380.00
LI2-H82E7-200ug
$1645.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    LILRB2, ILT4, LIR2, MIR10, CD85d

  • Source

    Biotinylated Human LILRB2 Protein, His,Avitag (LI2-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Val 461 (Accession # Q8N423-1).

    Predicted N-terminus: Gln 22

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  • Molecular Characterization

    LILRB2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 51.3 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    LILRB2 SDS-PAGE

    Biotinylated Human LILRB2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    LILRB2 SEC-MALS

    The purity of Biotinylated Human LILRB2 Protein, His,Avitag (Cat. No. LI2-H82E7) is more than 90% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     LILRB2 ELISA

    Immobilized Anti-LILRB2 Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human LILRB2 Protein, His,Avitag (Cat. No. LI2-H82E7) with a linear range of 0.3-20 ng/mL (QC tested).

    Protocol
  •  LILRB2 ELISA

    Immobilized Biotinylated Human LILRB2 Protein, His,Avitag (Cat. No. LI2-H82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-LILRB2 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).

    Protocol

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Introduction de fond

Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) is also known as CD85 antigen-like family member D (CD85d), Immunoglobulin-like transcript 4 (ILT-4), Monocyte / macrophage immunoglobulin-like receptor 10 (MIR-10), which is a member of the the subfamily B class of LIR receptors. LILRB2 is receptor for class I MHC antigens. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB2 competes with CD8A for binding to class I MHC antigens. LILRB2 / CD85d inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Leukocyte immunoglobulin-like receptor subfamily B member 2

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    11 Détails

  • Étape de développement la plus élevée:

    Phase 2 Clinical

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