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Biotinylated Mouse LILRB4 / CD85k / ILT3 Protein, His,Avitag™ (MALS verified)

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Numéro de produit/spécification
Prix
Qté
CDK-M82E3-25ug
$360.00
CDK-M82E3-200ug
$1420.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    LILRB4, ILT3, LIR5, CD85K, HM18

  • Source

    Biotinylated Mouse LILRB4 Protein, His,Avitag (CDK-M82E3) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Lys 238 (Accession # Q64281-1 ).

    Predicted N-terminus: Gly 24

    Request for sequence
  • Molecular Characterization

    LILRB4 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 27.6 kDa. The protein migrates as 37-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    LILRB4 SDS-PAGE

    Biotinylated Mouse LILRB4 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    LILRB4 SEC-MALS

    The purity of Biotinylated Mouse LILRB4 Protein, His,Avitag (Cat. No. CDK-M82E3) is more than 95% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     LILRB4 SPR

    Biotinylated Mouse LILRB4 Protein, His,Avitag (Cat. No. CDK-M82E3) immobilized on SA Chip can bind Mouse Apolipoprotein E Protein, His Tag (Cat. No. APE-M52H5) with an affinity constant of 565 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Introduction de fond

Leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) is also known as CD85 antigen-like family member K (CD85K), Immunoglobulin-like transcript 3 (ILT-3), Leukocyte immunoglobulin-like receptor 5 (LIR-5), Monocyte inhibitory receptor HM18, which belongs to the leukocyte immunoglobulin-like receptor (LIR) family. LILRB4 / CD85K contains 2 Ig-like C2-type (immunoglobulin-like) domains. CD85K is detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells. LILRB4 / CD85K is receptor for class I MHC antigens. CD85K recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles, involved in the down-regulation of the immune response and the development of tolerance. LILRB4 interferes with TNFRSF5-signaling and NF-kappa-B up-regulation and inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Leukocyte immunoglobulin-like receptor subfamily B member 4

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    7 Détails

  • Étape de développement la plus élevée:

    Phase 2 Clinical

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