Human Matriptase / ST14 Catalytic Domain Protein, His Tag (active enzyme)

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MAE-H5143-100ug
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MAE-H5143-1mg
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MAE-H5143
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Synonyms

Matriptase

Source

Human Matriptase / ST14 Catalytic Domain Protein, His Tag (MAE-H5143) is expressed from E. coli cells. It contains AA Gly 596 - Val 855 (Accession # NP_068813).

Predicted N-terminus: Met

Molecular Characterization

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 30.6 kDa. The protein migrates as 27-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Purity

>90% as determined by SDS-PAGE.

Formulation

Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, Trehalose, pH7.5 with glycerol as protectant.

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Shipping and Storage

This product is supplied and shipped on dry ice.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • The product MUST be stored at -70°C or lower upon receipt;
  • -70°C for 3 months under sterile conditions.

Background

Degrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site. Involved in the terminal differentiation of keratinocytes through prostasin (PRSS8) activation and filaggrin (FLG) processing; Cleaves various synthetic substrates with Arg or Lys at the P1 position and prefers small side-chain amino acids, such as Ala and Gly, at the P2 position.