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Biotinylated Human Mucin-13 Protein, Fc,Avitag™ (MALS verified)

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Numéro de produit/spécification
Prix
Qté
MU3-H82F3-25ug
$360.00
MU3-H82F3-200ug
$1565.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    Mucin-13, MUC13

  • Source

    Biotinylated Human Mucin-13 Protein, Fc,Avitag (MU3-H82F3) is expressed from human 293 cells (HEK293). It contains AA Thr 19 - Leu 421 (Accession # Q9H3R2).

    Predicted N-terminus: Thr 19

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  • Molecular Characterization

    Mucin-13 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 71.1 kDa. The protein migrates as 110-140 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    Mucin-13 SDS-PAGE

    Biotinylated Human Mucin-13 Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Mucin-13 SEC-MALS

    The purity of Biotinylated Human Mucin-13 Protein, Fc,Avitag (Cat. No. MU3-H82F3) is more than 90% and the molecular weight of this protein is around 200-245 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Mucin-13 ELISA

    Immobilized Biotinylated Human Mucin-13 Protein, Fc,Avitag (Cat. No. MU3-H82F3) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind MUC-13C14-hIgG1 with a linear range of 0.004-0.25 μg/mL (QC tested).

    Protocol

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Introduction de fond

Epithelial mucins, such as MUC13, are a family of secreted and cell surface glycoproteins expressed by ductal and glandular epithelial tissues (Williams et al., 2001 [PubMed 11278439]).[supplied by OMIM, Jul 2008]

Progrès de frontière

 
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