Product Details
Synonyms
Netrin receptor DCC, DCC, IGDCC1, netrin receptor DCC
Source
Human Netrin receptor DCC, His Tag (NEC-H52H3) is expressed from human 293 cells (HEK293). It contains AA Phe 32 - Asn 1097 (Accession # P43146-1).
Predicted N-terminus: Phe 32
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 119.6 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human Netrin receptor DCC, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Human Netrin receptor DCC, His Tag (Cat. No. NEC-H52H3) is more than 85% and the molecular weight of this protein is around 130-145 kDa verified by SEC-MALS.
Report
Bioactivity-SPR

Human Netrin-1 Protein immobilized on CM5 Chip can bind Human Netrin receptor DCC, His Tag (Cat. No. NEC-H52H3) with an affinity constant of 31.8 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
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