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Human NKG2D / CD314 Protein, Mouse IgG2a Fc Tag

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Numéro de produit/spécification
Prix
Qté
NKD-H5259-100ug
$340.00
NKD-H5259-1mg (500ug X 2)
$2245.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    NKG2D, CD314, KLRK1, NK cell receptor D

  • Source

    Human NKG2D, Mouse IgG2a Fc Tag (NKD-H5259) is expressed from human 293 cells (HEK293). It contains AA Ile 73 - Val 216 (Accession # P26718-1).

    Predicted N-terminus: Glu

    Request for sequence
  • Molecular Characterization

    NKG2D Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a mouse IgG2a Fc tag at the N-terminus.

    The protein has a calculated MW of 43.5 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.1 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    NKG2D SDS-PAGE

    Human NKG2D, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-ELISA

     NKG2D ELISA

    Immobilized Human MICA, His Tag (Cat. No. MIA-H5221) at 1 μg/mL (100 μL/well) can bind Human NKG2D, Mouse IgG2a Fc Tag (Cat. No. NKD-H5259) with a linear range of 0.02-0.313 μg/mL (QC tested).

    Protocol

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Introduction de fond

NKG2D is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors, also known as KLRK1, CD314, D12S2489E, KLR and killer cell lectin like receptor K1. NKG2D itself forms a homodimer whose ectodomains serve for ligand binding. NKG2D is a major recognition receptor for the detection and elimination of transformed and infected cells as its ligands are induced during cellular stress, either as a result of infection or genomic stress such as in cancer. In NK cells, NKG2D serves as an activating receptor, which itself is able to trigger cytotoxicity. The function of NKG2D on CD8+ T cells is to send co-stimulatory signals to activate them.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    NKG2-D type II integral membrane protein

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    10 Détails

  • Étape de développement la plus élevée:

    Phase 2 Clinical

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