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Product Details
Application
Synonyms
NY-ESO-1 TCR & CD3E & CD3D & CD3G & CD3Z
Source
Nanodisc-pro Human NY-ESO-1 TCR & CD3 Protein Complex, His,Flag Tag (NYZ-H52D3) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Ile 207 & Phe 22 - Lys 171 & Gln 23 - Asn 182 & Gln 22 - Arg 164 (Accession # P07766-1 & P04234-1 & P09693-1 & P20963-1).
Predicted N-terminus: Phe
Molecular Characterization
The protein has a calculated MW of 31.2 kDa & 33.4 kDa | 18.9 kDa & 22.9 kDa & 20.4 kDa & 18.0 kDa.
*The isotype control of empty/mock nanodisc (Cat. No. APO-H5143) is sold separately and not included in protein, you can follow the link for product information.
Structure
Nanodisc-pro membrane protein is wrapped by a piece of cell membrane and scaffold, the info of scaffold can be found in above red text "isotope type control...".
Synthesis Process
Nanodisc-pro membrane protein extraction is a gentle, non-disruptive process that directly isolates full-length transmembrane proteins together with their surrounding native phospholipids from cellular membranes, without breaking or disturbing the original membrane structure. This preserves the membrane protein's natural conformational landscape and native lipid environment, yielding stable nanodisc that retain cellular phospholipids and eliminate the need for detergent solubilization, with fully preserved biological activity—making them ideal for cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA), etc.
Formulation
Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Performance Data
Bioactivity-ELISA
Immobilized Human NY-ESO-1 TCR & CD3 Protein Complex, His,Flag Tag (Cat. No. NYZ-H52D3) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with a linear range of 0.2-3 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Complex Protein (Cat. No. HL1-H82E6) at 2 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human NY-ESO-1 TCR & CD3 Protein Complex, His,Flag Tag (Cat. No. NYZ-H52D3) with a linear range of 0.01-0.313 μg/mL (QC tested).
Protocol
Immobilized Human NY-ESO-1 TCR & CD3 Protein Complex, His,Flag Tag (Cat. No. NYZ-H52D3) at 5 μg/mL (100 μL/well) can bind FITC anti-human TCR α/β Antibody with a linear range of 0.01-0.625 μg/mL (Routinely tested).
Protocol
Immobilized Human NY-ESO-1 TCR & CD3 Protein Complex, His,Flag Tag (Cat. No. NYZ-H52D3) at 5 μg/mL (100 μL/well) can bind Llama Anti-CD3 with a linear range of 0.05-2 ng/mL (Routinely tested).
Protocol
Immobilized Human NY-ESO-1 TCR & CD3 Protein Complex, His,Flag Tag (Cat. No. NYZ-H52D3) at 1 μg/mL (100 μL/well) can bind Anti-BCMA×CD3 Antibody (Teclistamab Biosimilar) with a linear range of 0.2-63 ng/mL (Routinely tested).
Protocol
Immobilized Human NY-ESO-1 TCR & CD3 Protein Complex, His,Flag Tag (Cat. No. NYZ-H52D3) at 1 μg/mL (100 μL/well) can bind Anti-KLK2×CD3 Antibody (Pasritamig Biosimilar) with a linear range of 0.06-16 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) captured on CM5 chip via anti-mouse antibodies surface can bind Human NY-ESO-1 TCR & CD3 Protein Complex, His,Flag Tag (Cat. No. NYZ-H52D3) with an affinity constant of 2.72 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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