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Détails du produit
Synonyms
PDCD1, PD1, CD279, SLEB2
Source
Human PD-1 Protein, His Tag (PD1-H52H2) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization
Human PD-1 Protein, His Tag is designed and expressed as nanoparticles of approximately 25 nm diameter by displaying PD-1 ECD in native conformation. The nanoparticles each was coated with an average of 180 PD-1 ECD.
The nanoparticle technology allows directional and high-density display of human PD-1 on the surface of nanoparticles, thus greatly enhances the immune response in vivo to otherwise poorly immunogenic epitopes.
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.1 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.5 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS with glycine and sodium citrate, pH8.0 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human PD-1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Human PD-1 Protein, His Tag (Cat. No. PD1-H52H2) at 1 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Immobilized Human PD-1 Protein, His Tag (Cat. No. PD1-H52H2) at 1 μg/mL (100 μL/well) can bind Human PD-L1 Protein, Mouse IgG1 Fc Tag (Cat. No. PD1-H52A3) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Immobilized Human PD-1 Protein, His Tag (Cat. No. PD1-H52H2) at 1 μg/mL (100 μL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD2-H52A5) with a linear range of 1-2 ng/mL (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Programmed cell death protein 1
- Catégorie：
- Nombre de médicaments répertoriés：
22 Détails
- Nombre de médicaments cliniques：
193 Détails
- Étape de développement la plus élevée：
Approved
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