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Détails du produit
Synonyms
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Source
Human PD-L1, His Tag (PD1-H5229) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1 ).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.0 kDa. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Mass Spectrometry
Measured Molecular Mass
The molecular weight by mass spectrometry is 26018 Da (Routinely tested).
Sequence Coverage
Human PD-L1, His Tag(PD1-H5229) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving 100% sequence coverage (Routinely tested).
N-terminal Sequence Analysis
Phe-Thr-Val-Thr-Val-Pro-Lys-Asp-Leu-Tyr-Val-Val-Glu-Tyr-Gly
(Routinely tested)
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human PD-L1, His Tag (Cat. No. PD1-H5229) is more than 95% and the molecular weight of this protein is around 30-50 kDa verified by SEC-MALS.
Report
Mass Spectrometry
ESI‑MS analysis of Human PD-L1, His Tag (Cat. No. PD1-H5229), showing a major peak at 26018 Da. The minor peak following the major peak is a matrix‑associated artifact of the ESI‑MS (Routinely tested).
Bioactivity-ELISA
Immobilized Human PD-L1, His Tag (Cat. No. PD1-H5229) at 1 μg/mL (100 μL/well) can bind Anti-Human PD-L1 MAb (Human IgG1) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Immobilized Human PD-L1, His Tag (Cat. No. PD1-H5229) at series of concentration on MonoRabᵀᴹ Anti-His Tag (C-term) Antibody precoated (0.1 μg/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag (Cat. No. PD1-H5255) at 5 μg/mL (100 μL/well) with a linear range of 10-78 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human PD-1, Fc Tag (Cat. No. PD1-H5257) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 3.6 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Anti-Human PD-L1 Mab (Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 0.286 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human PD-1, Fc Tag, low endotoxin (Cat. No. PD1-H5257) on ProteinA Biosensor, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 5.3 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Anti-Human PD-L1 MAb (Human IgG1) on AHC Biosensor, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 1.61 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Avis des utilisateurs Publier un commentaire
A***n2024-10-18
J'aimeWe order at Acrobiosystems for ELISA/ECL reagents. Up to now, we are happy with their products (quality) and the support they provide to us. It is quick to have a quote and very fast shipment.- 1***32022-08-10 J'aimeThis is the third time we’ve purchased this antigen with good batch-to-batch stability and high experimental reproducibility. Both of our ELISA assays and activity experiments are proceeding smoothly and I hope the experiment goes well for the antibody screening as well! :)
- Z***N2022-02-09 J'aimeUsing the PDL1 protein provided by Acro Biosystems, I am trying to screen for aptamers that can specifically bind to PDL1 and further use that aptamer for personalized cancer immunotherapy. Though the work is ongoing, I am impressed by the quality of the product and services offered by Acro Biosystems. Will share more about our work afterwards. If you plan to try PDL1 protein for your research, I will definitely recommend this one.
FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Programmed death-ligand 1
- Catégorie：
- Nombre de médicaments répertoriés：
12 Détails
- Nombre de médicaments cliniques：
176 Détails
- Étape de développement la plus élevée：
Approved
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