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Product Details
Synonyms
Fletcher factor, kallikrein B, plasma (Fletcher factor) 1, kininogenin, KLK3 plasma kallikrein, KLKB1, plasma kallikrein heavy chain, plasma kallikrein light chain, Plasma Kallikrein, Plasma Prekallikrein, PPK, KLKB1
Source
Cynomolgus Plasma Kallikrein Protein, His Tag (KL1-C52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - Ala 638 (Accession # A0A2K5VTJ9).
Predicted N-terminus: Gly 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 71.0 kDa. The protein migrates as 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
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Performance Data
SDS-PAGE
Cynomolgus Plasma Kallikrein Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus Plasma Kallikrein Protein, His Tag (Cat. No. KL1-C52H3) is more than 95% and the molecular weight of this protein is around 75-95 kDa verified by SEC-MALS.
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Bioactivity
Measured by its ability to cleave a fluorogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >1,700 pmol/min/μg (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Plasma kallikrein
- Category：
- Approved Drugs：
4 Details
- Drugs in Clinical Trials：
12 Details
- Highest Development Stage：
Approved
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