Human PTH1R / PTHR1 Protein, His Tag, low endotoxin (MALS verified)

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Related Products: Cell line CHEK-ATF194
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PTR-H5222-100ug
$260.00
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PTR-H5222-1mg (250ug X 4)
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PTR-H5222
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Synonyms

PTH1R, PTHR, PTHR1

Source

Human PTH1R, His Tag (PTR-H5222) is expressed from human 293 cells (HEK293). It contains AA Asp 27 - Gly 188 (Accession # Q03431-1).

Predicted N-terminus: Asp 27

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 19.6 kDa. The protein migrates as 27-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>85% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Introduction de fond

Parathyroid hormone/parathyroid hormone-related peptide receptor is also known as Parathyroid hormone 1 receptor (PTH1R), PTHR1, PTH/PTHrP type I receptor (PTH/PTHr receptor), which belongs to the G-protein coupled receptor 2 family. PTH1R / PTHR1 is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.