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Détails du produit
Synonyms
PTK7, CCK4, CCK-4, Tyrosine-protein kinase-like 7, Protein-tyrosine kinase 7, Protein-tyrosine kinase 7
Source
Biotinylated Human PTK7, His,Avitag (PT7-H82E5) is expressed from human 293 cells (HEK293). It contains AA Ala 31 - Thr 704 (Accession # Q13308-1).
Predicted N-terminus: Ala 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 78.2 kDa. The protein migrates as 105-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Biotinylated Human PTK7, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human PTK7, His,Avitag (Cat. No. PT7-H82E5) is more than 95% and the molecular weight of this protein is around 95-130 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human PTK7, His,Avitag (Cat. No. PT7-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Cofetuzumab Pelidotin with a linear range of 0.06-1 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Cofetuzumab captured on Protein A Chip can bind Biotinylated Human PTK7, His,Avitag (Cat. No. PT7-H82E5) with an affinity constant of 4.88 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Protein-tyrosine kinase 7
- Catégorie：
- Nombre de médicaments répertoriés：
0 Détails
- Nombre de médicaments cliniques：
8 Détails
- Étape de développement la plus élevée：
Phase 2 Clinical
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