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Détails du produit
Synonyms
Rep 68
Source
Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2) Rep 68, His Tag (RE8-A5243) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Leu 536 (Accession # P03132-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 62.8 kDa. The protein migrates as 75-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2) Rep 68, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2) Rep 68, His Tag (Cat. No. RE8-A5243) at 1 μg/mL (100 μL/well) can bind Monoclonal Mouse anti‑Adeno‑Associated Virus AAV2 Rep68 Antibody with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
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T***d2025-04-09
J'aimeI used the Rep 68 Protein for a Western Blot positive control and it worked perfectly ! The signal I had was strong with a short time of exposure, and it was one clean band. Furthermore, I only used a few ng of the sample to load the Western Blot gel, so I can do many many Western Blots with a single tube. I also found it easy to handle as the reconstitution was clear.
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