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Product Details
Ribonuclease R (RNase R) is a magnesium-dependent 3′→ 5′ exoribonuclease that digests linear RNAs. RNase R does not digest lariat or circular RNA structures.
Application
Removal of precursor linear RNA after circularisation of RNA
Alternative splicing studies
Gene expression studiesUnit Definition
One unit is the amount of enzyme that will converts 1 µg of poly(A) into acid-soluble nucleotides in 10 minutes at 37 °C under standard assay conditions.
Purity
>95% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 20mM Tris, 150mM NaCl, 0.1mM EDTA, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
This product is stable after storage at:
- The product MUST be stored at -20°C or lower upon receipt.
- -20°C for 6 months under sterile conditions.
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Affichage des données
SDS-PAGE
The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity
Mixing 1ug ssRNA with 0.6-3U RNase R at 37°C, 30min then electrophoresis detection, it was observed that the ssRNA bands became weak or even disappeared.
1 ug circRNA substrate was mixed with 0.5-2 U RNase R at 37 °C, 30min then electrophoresis detected, there was no significant change in circRNA.
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