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SPARC, BM-40, ON, Osteonectin, Basement-membrane protein 40
Human SPARC protein, His Tag (SPC-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 18 - Ile 303 (Accession # P09486).
Predicted N-terminus: Ala 18
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 34.6 kDa. The protein migrates as 40-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method / rFC method.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.