SARS S protein (R667A, K968P, V969P), His Tag (MALS verified)

Spike, S protein, Spike glycoprotein, S glycoprotein

SARS S protein (R667A, K968P, V969P), His Tag (SPN-S52H6) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P)). The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.

Less than 1.0 EU per μg by the LAL method / rFC method.

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 0.2 M Sodium citrate, pH5.5 with trehalose as protectant.

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Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

• -20°C to -70°C for 12 months in lyophilized state;
• -70°C for 3 months under sterile conditions after reconstitution.