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Bat SARS-like coronavirus RsSHC014 Spike RBD Protein, His Tag (MALS verified)

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Numéro de produit/spécification
Prix
Qté
SPD-S52Hd-100ug
$336.00
SPD-S52Hd-1mg
$2184.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD

  • Source

    Bat SARS-like coronavirus RsSHC014 Spike RBD Protein, His Tag (SPD-S52Hd) is expressed from human 293 cells (HEK293). It contains AA Arg 307 - Gln 524 (Accession # AGZ48806.1).

    Predicted N-terminus: Arg 307

    Request for sequence
  • Molecular Characterization

    Spike RBD Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.2 kDa. The protein migrates as 32-37 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    Spike RBD SDS-PAGE

    Bat SARS-like coronavirus RsSHC014 Spike RBD Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Spike RBD SEC-MALS

    The purity of Bat SARS-like coronavirus RsSHC014 Spike RBD Protein, His Tag (Cat. No. SPD-S52Hd) is more than 90% and the molecular weight of this protein is around 25-40 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Spike RBD ELISA

    Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind Bat SARS-like coronavirus RsSHC014 Spike RBD Protein, His Tag (Cat. No. SPD-S52Hd) with a linear range of 0.002-0.125 μg/mL (QC tested).

    Protocol

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Introduction de fond

The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Progrès de frontière

 
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