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Détails du produit
Synonyms
Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD
Source
SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (SPD-C522g) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2; GISAID clade: GRA; Nextstrain clade: 21L).
Predicted N-terminus: Arg 319
Request for sequenceMolecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.7 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (Cat. No. SPD-C522g) is more than 90% and the molecular weight of this protein is around 32-48 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (Cat. No. SPD-C522g) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) with a linear range of 0.8-13 ng/mL (QC tested).
Protocol
Immobilized SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (Cat. No. SPD-C522g) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.4-13 ng/mL (Routinely tested).
Protocol
Serial dilutions of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122) (Trehalose free) (Cat. No. S1N-M12A1) can inhibit SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (MALS verified) (Cat. No. SPD-C522g) from binding Human ACE2. The half maximal inhibitory concentration (IC50) is 1.844 μg/mL (Routinely tested).
Protocol
Serial dilutions of Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (MALS verified) (Cat. No. SPD-M265) can inhibit SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (MALS verified) (Cat. No. SPD-C522g) from binding Human ACE2. The half maximal inhibitory concentration (IC50) is 2.038 μg/mL (Routinely tested).
Protocol
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