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Product Details
Synonym
Streptavidin, SA
Source
Streptavidin Protein-Acridinium ester (STN-NA114) is Acridinium ester chemically conjugated Streptavidin expressed from E. coli cells.
Molecular Characterization
Streptavidin carries no "tag". The protein has a calculated MW of 13.8 kDa. The protein migrates as 15-16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
The protein is designed as a tetramer.
Labeling
Acridinium ester, can react with the primary amino group of protein. Under alkaline conditions, NHS is replaced as the leaving group, and the protein forms a stable amide bond with Acridinium ester.
Protein Ratio
Passed as determined by binding MPCLIA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH6.3 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Streptavidin Protein-Acridinium ester on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Streptavidin Protein-Acridinium ester (Cat. No. STN-NA114) is more than 90% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.
Report
Bioactivity-MPCLIA
Immobilized 0.04 μg/Test of Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4) to the Streptavidin Protein-Acridinium ester (Cat. No. STN-NA114, 0.008 μg/Test), incubated with 20 μL/Test of Human PD-L1 Protein, Mouse IgG1 Fc Tag (Cat. No. PD1-H52A3) at increasing concentration coupled to Anti-Mouse IgG Magnetic Beads (10 μg beads/Test). Detection was performed with sensitivity of 7.8 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
Immobilized 0.04 μg/Test of Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82E9) to the Streptavidin Protein-Acridinium ester (Cat. No. STN-NA114, 0.008 μg/Test), incubated with 20 μL/Test of Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) (Cat. No. S1N-M12A1) at increasing concentration coupled to Protein G Magnetic Beads (10 μg beads/Test). Detection was performed with sensitivity of 0.244 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (Routinely tested).
Protocol
Immobilized 0.04 μg/Test of Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82E9) to the Streptavidin Protein-Acridinium ester (Cat. No. STN-NA114, 0.008 μg/Test), incubated with 20 μL/Test of Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) (Cat. No. S1N-M12A1) at increasing concentration coupled to Anti-Human IgG Magnetic Beads (10 μg beads/Test). Detection was performed with sensitivity of 7.8 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (Routinely tested).
Protocol
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