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Streptavidin Protein-Cy5

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Numéro de produit/spécification
Prix
Qté
STN-NC113-100ug
$80.00
STN-NC113-1mg (200ug X 5)
$165.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    Streptavidin, SA

  • Source

    Streptavidin Protein-Cy5 (STN-NC113) is expressed from E. coli cells.

  • Molecular Characterization

    This protein carries no "tag".

    The protein has a calculated MW of 13.8 kDa.

  • Conjugate

    Cy5

    Excitation Wavelength: 651 nm

    Emission Wavelength: 670 nm

  • Labeling

    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with Cy5 using standard chemical labeling method. The residual Cy5 is removed by molecular sieve treatment during purification process.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Affichage des données

  • Bioactivity-FACS

     Streptavidin FACS

    5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 20 ug/mL Biotinylated Human CD19 (20-291) Protein, Fc, Avitag™, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-Cy5 (Cat. No. STN-NC113) and analyzed with FACS. Cy5 signal was used to evaluate the binding activity (QC tested).

    Protocol

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Introduction de fond

The Streptavidin Protein Europium chelate is a universal tool for TR-FRET assays that can bind biotinylated molecules. This product uses high-purity streptavidin (SA) covalently conjugated with Eu³⁺ chelate, and it can be used in combination with other Acceptors directly or indirectly labeled with fluorescent dyes. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.

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  • Informations de base sur le produit
  • Détails du produit
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