T4 RNA Ligase 1(10U/ul)

Product Details

T4 RNA Ligase 1 catalyzes the ligation of a 5´ phosphoryl-terminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acid acceptor through the formation of a 3´ → 5´ phosphodiester bond with hydrolysis of ATP to AMP and PPi.

Application

Joining RNA to RNA
Synthesis of oligoribonucleotides and oligodeoxyribonucleotides
Specific modifications of tRNAs
RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate
Site-specific generation of composite primers for PCR

Unit Definition

One unit is defined as the amount of enzyme required to convert 1 nanomole of 5´-[32P]rA16 into a phosphatase-resistant form in 30 minutes at 37°C.

Purity

>90% as determined by SDS-PAGE.

Notes

The coexistence of PEG promotes the response without changing the specificity of the reaction.

Formulation

Supplied as 0.2 μm filtered solution in 10 mM Tris, 0.1 mM EDTA, 1 mM DTT, pH 7.4 with glycerol as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

This product is stable after storage at:

1. This product is stable for up to 12 months at -20℃ from date of receipt.