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Mouse TIGIT Protein, Fc Tag (MALS verified)

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Numéro de produit/spécification
Prix
Qté
TIT-M5257-50ug
$360.00
TIT-M5257-1mg (500ug X 2)
$2245.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    TIGIT, VSIG9, VSTM3

  • Source

    Mouse TIGIT, Fc Tag (TIT-M5257) is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Thr 143 (Accession # NP_001139797.1).

    Predicted N-terminus: Gly 26

    Request for sequence
  • Molecular Characterization

    TIGIT Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 39.5 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    TIGIT SDS-PAGE

    Mouse TIGIT, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    TIGIT SEC-MALS

    The purity of Mouse TIGIT, Fc Tag (Cat. No. TIT-M5257) is more than 95% and the molecular weight of this protein is around 80-105 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     TIGIT ELISA

    Immobilized Mouse CD155, mouse IgG2a Fc tag (Cat. No. CD5-M5254) at 2 μg/mL (100 μL/well) can bind Mouse TIGIT, Fc Tag (Cat. No. TIT-M5257) with a linear range of 1-16 ng/mL (QC tested).

    Protocol

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Introduction de fond

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    T cell immunoglobulin and ITIM domains

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    41 Détails

  • Étape de développement la plus élevée:

    Phase 3 Clinical

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