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Human TRAIL R3 protein, Fc Tag (MALS verified)

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Numéro de produit/spécification
Prix
Qté
TR3-H52F3-100ug
$350.00
TR3-H52F3-1mg
$2310.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    CD263, DCR1, DCR1-TNFR, LIT, TRAIL-R3, TRAILR3, TRID

  • Source

    Human TRAIL R3 protein, Fc Tag (TR3-H52F3) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Pro 235 (Accession # O14798).

    Predicted N-terminus: Ala 26

    Request for sequence
  • Molecular Characterization

    TRAIL R3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 48.6 kDa. The protein migrates as 65-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    TRAIL R3 SDS-PAGE

    Human TRAIL R3 protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    TRAIL R3 SEC-MALS

    The purity of Human TRAIL R3 protein, Fc Tag (Cat. No. TR3-H52F3) is more than 95% and the molecular weight of this protein is around 120-145 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     TRAIL R3 ELISA

    Immobilized Human TRAIL R3 protein, Fc Tag (Cat. No. TR3-H52F3) at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) with a linear range of 1-78 ng/mL (QC tested).

    Protocol

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Introduction de fond

Tumor necrosis factor receptor superfamily member 10C (TNFRSF10C) is also known as Decoy receptor 1 (DcR1), TNF-related apoptosis-inducing ligand receptor 3 (TRAIL receptor 3 or TRAIL-R3), CD263. TNFRSF10C is a decoy receptor for TRAIL (tumor necrosis factor-related apoptosis-inducing ligand), a member of the tumor necrosis factor (TNF) ligand family. This receptor is not capable of inducing apoptosis (lacks a cytoplasmic death domain), and is thought to function as an antagonistic receptor that protects cells from TRAIL-induced apoptosis.

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