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Product Details
Synonyms
TSLP
Source
Biotinylated Human TSLP (R127A, R130A) Protein, Fc,Avitag (TSP-H82F4) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1 (R127A, R130A)).
Predicted N-terminus: Tyr 29
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 42.9 kDa. The protein migrates as 53-58 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human TSLP (R127A, R130A) Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human TSLP (R127A, R130A) Protein, Fc,Avitag (Cat. No. TSP-H82F4) is more than 85% and the molecular weight of this protein is around 110-135 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP (R127A, R130A) Protein, Fc,Avitag (Cat. No. TSP-H82F4) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Monoclonal Anti-Human TSLP Antibody, Human IgG2 at 1 μg/mL (100 μL/well) can bind Biotinylated Human TSLP (R127A, R130A) Protein, Fc,Avitag (Cat. No. TSP-H82F4) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Human TSLP Protein, Fc,Avitag (Cat. No. TSP-H82F4) immobilized on SA Chip can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with an affinity constant of 146 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Biotinylated Human TSLP Protein, Fc,Avitag (Cat. No. TSP-H82F4) immobilized on SA Chip can bind Monoclonal Anti-Human TSLP Antibody, Human IgG2 with an affinity constant of 0.907 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
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- Highest Development Stage：
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