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Product Details
Synonyms
FIGF, VEGFD
Source
Human VEGF-D Protein, His Tag (VED-H52H3) is expressed from human 293 cells (HEK293). It contains AA Phe 93 - Ser 201 (Accession # O43915).
Predicted N-terminus: Phe 93
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 18.0 kDa. The protein migrates as 25-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
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Performance Data
SDS-PAGE
Human VEGF-D Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human VEGF-D Protein, His Tag (Cat. No. VED-H52H3) is more than 90% and the molecular weight of this protein is around 40-56 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) at 5 μg/mL (100 μL/well) can bind Human VEGF-D Protein, His Tag (Cat. No. VED-H52H3) with a linear range of 0.001-5 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) captured on Protein A Chip can bind Human VEGF-D Protein, His Tag (Cat. No. VED-H52H3) with an affinity constant of 38.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Vascular endothelial growth factor D
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 2 Clinical
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