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Product Details
Application
In in vivo CAR research, the endocytic capacity of tLNP is a key factor for evaluation of the delivery efficiency. Our pH-sensitive Anti-Human IgG Labeling Reagent (Cat. IGG-ZFS307) specifically binds to the Fc region of human IgG antibodies, and the pH-sensitive dye can significantly enhance the fluorescence signal in an acidic environment. This enables FACS analysis to evaluate the endocytic efficiency of IgG-format antibody-conjugated LNPs.
Features
- High signal-to-noise ratio – Strong fluorescence signal with minimal background
- Fc-specific targeting – Preserves antibody–antigen interaction
- Application validated – FACS-ready for tLNP uptake studies
Specificity
Specifically binds to the Fc region of human IgG without recognizing Fab fragments or mouse IgG isotypes.
Detection Wavelength
Excitation Wavelength: 643 nm
Emission Wavelength: 660 nmStorage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -20°C to -70°C for 3 months after reconstitution.
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Performance Data
FACS Data
Anti-CD20 Abs and Human IgG1 isotype control were labeled with pH Sensitive Anti-Human IgG Labeling Reagent (Cat. No. IGG-ZFS307). Raji cells were treated with Anti-CD20 Abs-Internalization Detection Reagent conjugate and Isotype control-Internalization Detection Reagent conjugate separately for 2 hours, then analysis by Flow cytometric. APC signal was used to evaluate the activity (QC tested).
Protocol
tLNP-hCD8 Ab and LNP-Human IgG1 Kappa Isotype Control were labeled with pH Sensitive Anti-Human IgG Labeling Reagent (Cat. No. IGG-ZFS307). CD3+ T cells were separately treated with pH-sensitive Dye-Labeled tLNP-hCD8 Ab or isotype control conjugates for 4 hours, then analysis by Flow cytometric. APC signal was used to evaluate the activity (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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