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Product Details
Application
In in vivo CAR research, the endocytic capacity of tLNP is a key factor for evaluation of the delivery efficiency. Our pH-sensitive Anti-Human IgG Labeling Reagent (Cat. IGG-ZFS307) specifically binds to the Fc region of human IgG antibodies, and the pH-sensitive dye can significantly enhance the fluorescence signal in an acidic environment. This enables FACS analysis to evaluate the endocytic efficiency of IgG-format antibody-conjugated LNPs.
Features
- High signal-to-noise ratio – Strong fluorescence signal with minimal background
- Fc-specific targeting – Preserves antibody–antigen interaction
- Application validated – FACS-ready for tLNP uptake studies
Specificity
Specifically binds to the Fc region of human IgG without recognizing Fab fragments or mouse IgG isotypes.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -20°C to -70°C for 3 months after reconstitution.
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Performance Data
FACS Data
Anti-CD20 Abs and Human IgG1 isotype control were labeled with pH Sensitive Anti-Human IgG Labeling Reagent (Cat. No. IGG-ZFS307). Raji cells were treated with Anti-CD20 Abs-Internalization Detection Reagent conjugate and Isotype control-Internalization Detection Reagent conjugate separately for 2 hours, then analysis by Flow cytometric. APC signal was used to evaluate the activity (QC tested).
Protocol
tLNP-hCD8 Ab and LNP-Human IgG1 Kappa Isotype Control were labeled with pH Sensitive Anti-Human IgG Labeling Reagent (Cat. No. IGG-ZFS307). CD3+ T cells were separately treated with pH-sensitive Dye-Labeled tLNP-hCD8 Ab or isotype control conjugates for 4 hours, then analysis by Flow cytometric. APC signal was used to evaluate the activity (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
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