CD3E & CD3D

CD3E & CD3D

CD3E & CD3D Product List

Species
  • Human(13)
  • Cynomolgus(4)
  • Mouse(3)
  • Rabbit(2)
  • Canine(1)
  • Rat(1)
  • Sus scrofa (Pig)(1)
Tag
  • His Tag(9)
  • His Tag & Fc Tag & Flag Tag(4)
  • His Tag & Flag Tag(4)
  • His Tag & Avi Tag(3)
  • Llama IgG Fc Tag(3)
  • Fc Tag(1)
  • His Tag & Fc Tag & Avi Tag & Flag Tag(1)
Host
  • HEK293(25)
Conjugate
  • Unconjugated(15)
  • Biotin-labeled(5)
  • AF488 Labeled(1)
  • AF647 Labeled(1)
  • APC Labeled(1)
  • HRP Labeled(1)
  • PE Labeled(1)
Grade
  • RUO(25)
Low Endotoxin
  • Yes(9)
  • No(16)
MoleculeCat. No.Product NameSpeciesHostStructurePurityActivitySize&Price
CD3E & CD3D
Human CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag Free
Human
HEK293
HPLCSDS-PAGE
SPR

$1880.00

250ug
CD3E & CD3D
Human CD3 epsilon&CD3 delta Heterodimer Protein, Fc Tag&Fc Tag, low endotoxin (MALS verified)
Human
HEK293
MALSSDS-PAGE
ELISA

$415.00

100ug
CD3E & CD3D
Human CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag Free, low endotoxin (Mass Spec & MALS verified)
Human
HEK293
MSMALSSDS-PAGE
ELISASPR

$435.00

50ug
CD3E & CD3D
HOTAvitag
CDD-C82W6
Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)
Cynomolgus
HEK293
MALSSDS-PAGE
ELISASPRBLI

$460.00

25ug
CD3E & CD3D
HOTAvitag
CDD-H82W6
Biotinylated Human CD3 epsilon&CD3 delta Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)
Human
HEK293
MALSSDS-PAGE
ELISASPRTR-FRET

$470.00

25ug
CD3E & CD3D
Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin
Cynomolgus
HEK293
SDS-PAGE
ELISA

$380.00

100ug
CD3E & CD3D
Canine CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag Free (MALS verified)
Canine
HEK293
MALSSDS-PAGE

$390.00

100ug
CD3E & CD3D
Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag, low endotoxin (Mass Spec & MALS verified)
Cynomolgus
HEK293
MALSSDS-PAGE
ELISASPRBLI

$435.00

100ug
CD3E & CD3D
Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)
Cynomolgus
HEK293
MALSSDS-PAGE
ELISABLI

$380.00

100ug
CD3E & CD3D
Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin
Human
HEK293
SDS-PAGE
ELISA

$395.00

100ug
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FAQ

What binding and bioactivity data are available for the CD3 epsilon/delta proteins?

Binding and bioactivity data are available across multiple assay platforms, including ELISA, SPR and TR-FRET. For example, CDD-H82W6 binds the reference anti-CD3 antibodies OKT3 (by ELISA and SPR, KD 22.5 nM) and UCHT1 (by ELISA), and supports detection of CD3×BCMA bispecific T-cell engagers in human serum quantitation and TR-FRET assays.

How is the CD3 epsilon/delta heterodimer quality verified?

Heterodimer integrity and purity are confirmed by SEC-MALS (e.g., CDD-H82W6 purity >95% with a molecular weight consistent with the 1:1 heterodimer), and binding activity is validated by ELISA and SPR against reference anti-CD3 antibodies such as OKT3 and UCHT1.

Which CD3 epsilon/delta format should I choose for my assay?

It depends on the detection platform. For streptavidin-based capture assays, choose a biotinylated construct (CDD-H82W6 for human, CDD-C82W6 for cynomolgus). For label-free SPR/BLI, Fc-tagged versions such as CDD-H5255 are suitable. For flow-based binding, fluorescent conjugates such as APC-labeled CDD-HA2H3 or AF647-labeled CDD-HA2W4 are available.

Should I use a CD3 epsilon/delta heterodimer or a CD3 epsilon monomer for binding studies?

A CD3 epsilon monomer is a convenient choice for antibodies that recognize linear or monomeric epitopes. However, some anti-CD3 antibodies recognize conformational epitopes formed when CD3 epsilon associates with CD3 delta; for those clones, the epsilon/delta heterodimer more closely reproduces the native epitope. The choice depends on your antibody's epitope specificity.

What is the CD3 epsilon/delta heterodimer?

The CD3 epsilon/delta heterodimer is a recombinant protein in which the extracellular domains of CD3 epsilon and CD3 delta are co-expressed and assemble into a native-like heterodimer. It reproduces the dimeric epitope recognized by many anti-CD3 antibodies and is widely used to evaluate the CD3-binding arm of T-cell-engaging bispecific antibodies.
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