IL-2 R alpha

Yeast cells expressing and displaying different IL2 variants were incubated 1h in the presence of CD25 protein with His tag (Human Il-2 R alpha Acrobiosystems #ILA-H52H9) at a final concentration of 4µg/ml. Next, cells were washed and incubated with a mouse Anti-His-PE. The yeast cells were washed and were analyzed using FACS. We observed detectable amounts of fluorescence bound to yeast cells displaying specific IL2 variants confirming that CD25 could bind to certain constructs and providing evidence that certain IL2 variants were correctly folded.

I purchased the company’s mouse-derived CD25 protein for the evaluation of CD25 antibody affinity. It achieved a high sensitivity detection with a small amount! Very satisfied, will come back to purchase more if needed!