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Détails du produit
Source
Monoclonal Anti-NMTi Antibody, Rabbit IgG (M1E05) is a rabbit monoclonal antibody recombinantly expressed in HEK293 cells.
Antibody Type
Recombinant Monoclonal
Clone
M1E05
Isotype
Rabbit IgG, Kappa
Host Species
Rabbit
Immunogen
NMTi-BSA
Specificity
Specifically recognizes NMTi.
Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Bioactivity-ELISA
Immobilized Monoclonal Anti-NMTi Antibody, Rabbit IgG (M1E05) (Cat. No. NMI-MY2365) at 1 μg/mL (100 μL/well) can bind ADC-NMTi with a linear range of 0.5-16 ng/mL (QC tested).
Protocol
Serial dilutions of NMTi, N-Myristoyltransferase inhibitor were added into Monoclonal Anti-NMTi Antibody, Rabbit IgG (M1E05) (Cat. No. NMI-MY2365): ADC-NMTi binding reactions. The half maximal inhibitory concentration (IC50) is 1.27 μg/mL (Routinely tested).
Protocol
Cross Verification
ELISA binding of Monoclonal Anti-NMTi Antibody, Rabbit IgG (M1E05) (Cat. No. NMI-MY2365) with ADC-NMTi, Trastuzumab Deruxtecan, Sacituzumab Govitecam, Disitamab Vedotin (RC48), DM1 Antibody Human IgG and IgG1-MMAF conjugated antibody respectively.
The coating antibody was Monoclonal Anti-NMTi Antibody, Rabbit IgG (M1E05) (Cat. No. NMI-MY2365), used at 1 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including ADC-NMTi, Trastuzumab Deruxtecan, Sacituzumab Govitecam, Disitamab Vedotin (RC48), DM1 Antibody Human IgG and IgG1-MMAF conjugated antibodies used at 0.5 μg/mL concentration. The secondary antibody was HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8), mAb (Acro, Cat. No. IGG-LY69) used at 1:10000 concentration.
Monoclonal Anti-NMTi Antibody, Rabbit IgG (M1E05) (Cat. No. NMI-MY2365) is specific to ADC-NMTi, and has no cross-reactivity with Trastuzumab Deruxtecan, Sacituzumab Govitecam, Disitamab Vedotin (RC48), DM1 Antibody Human IgG and IgG1-MMAF (Routinely tested).
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