HRP conjugated Monoclonal Anti-PBD Antibody, Rabbit IgG (M1D08)

Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells
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PBD-PLMY2221-100ug
$370.00
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PBD-PLMY2221-1mg
$2,388.00
PBD-PLMY2221
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Source

HRP conjugated Monoclonal Anti-PBD Antibody, Rabbit IgG (M1D08) is a recombinant rabbit monoclonal antibody expressed in HEK293 cells.

Antibody Type

Recombinant Monoclonal

Clone

M1D08

Isotype

Rabbit IgG, Kappa

Host Species

Rabbit

Immunogen

PBD-BSA

Conjugate

HRP conjugated

Specificity

Specifically recognizes PBD.

Application
Application
Recommended Use
ELISA
1:600-1:6000

* Recommended dilutions are for reference only. Optimal working concentrations may vary depending on your consumables, reagents, and assay conditions. We recommend optimizing for your specific system. No warranty is made regarding performance at any specific dilution.

Purification

Protein A purified.

Concentration

Please refer to the Certificate of Analysis (CoA).

Form

Lyophilized

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

Shipping

Lyophilized product is shipped at ambient temperature.

Storage

For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
Notices

Product Specific Notices: For research use only.

Introduction de fond

Pyrrolobenzodiazepine (PBD) dimer, is a new generation of cytotoxic payload used in antibody-drug conjugates (ADCs). The PBD dimer binds to the minor groove of DNA to form effective cytotoxic DNA interstrand crosslinks, which can block cell division and kill cancer cells. This mechanism of action utilizes a completely different cellular target from that of tubulin inhibitors, as well as a different DNA damage pattern from other DNA-targeting payloads. Anti-PBD antibody is a rabbit monoclonal antibody specially reacts with PBD, which is more sensitive than mouse antibody. The anti-PBD antibody is a useful reagent in PK assay to determine conjugated antibodies.