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Détails du produit
Source
Monoclonal Anti-PTX Antibody, Rabbit IgG1 (M1F06) is a rabbit monoclonal antibody recombinantly expressed in HEK293 cells.
Antibody Type
Recombinant Monoclonal
Clone
M1F06
Isotype
Rabbit IgG, Kappa
Host Species
Rabbit
Immunogen
PTX-BSA
Specificity
Specifically recognizes PTX.
Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Affichage des données
Bioactivity-ELISA
Immobilized Monoclonal Anti-PTX Antibody, Rabbit IgG1 (M1F06) (Cat. No. PTX-MY2606) at 1 μg/mL, add Anti-HER2 mAb-Paclitaxel ADC in the 100% Human Serum and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized Monoclonal Anti-PTX Antibody, Rabbit IgG1 (M1F06) (Cat. No. PTX-MY2606) at 1 μg/mL, add Anti-HER2 mAb-Paclitaxel ADC in the 0.5% BSA and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).
Protocol
Immobilized Monoclonal Anti-PTX Antibody, Rabbit IgG1 (M1F06) (Cat. No. PTX-MY2606) at 1 μg/mL (100 μL/well) can bind Anti-HER2 mAb-Paclitaxel ADC with a linear range of 0.5-31 ng/mL (Routinely tested).
Protocol
Cross Verification
ELISA binding of Monoclonal Anti-PTX Antibody, Rabbit IgG1 (M1F06) (Cat. No. PTX-MY2606) with Anti-HER2 mAb-Paclitaxel ADC, Tusamitamab Ravtansine, Trastuzumab Deruxtecan, Sacituzumab Govitecam, Disitamab Vedotin (RC48), ADC-Duocarmycin, and ADC-NMTi conjugated antibody respectively.
The coating antibody was Monoclonal Anti-PTX Antibody, Rabbit IgG1 (M1F06) (Cat. No. PTX-MY2606), used at 1 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including Anti-HER2 mAb-Paclitaxel ADC, Tusamitamab Ravtansine, Trastuzumab Deruxtecan, Sacituzumab Govitecam, Disitamab Vedotin (RC48), ADC-Duocarmycin, and ADC-NMTi conjugated antibodies used at 0.5 μg/mL concentration. The secondary antibody was HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8), mAb (Acro, Cat. No. IGG-LY69) used at 1:10000 concentration.
Monoclonal Anti-PTX Antibody, Rabbit IgG1 (M1F06) (Cat. No. PTX-MY2606) is specific to Anti-HER2 mAb-Paclitaxel ADC and has no cross-reactivity with Tusamitamab Ravtansine, Trastuzumab Deruxtecan, Sacituzumab Govitecam, Disitamab Vedotin (RC48), ADC-Duocarmycin and ADC-NMTi (Routinely tested).
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