HRP conjugated Monoclonal Anti-SN38 Antibody, Mouse IgG1

  • Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells
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SN8-PLM685-25ug
$800.00
In Stock - Delivery in 1-2 business days
SN8-PLM685-200ug
$5330.00
In Stock - Delivery in 2 weeks
Total des articles Montant du produit$ 0

Détails du produit

  • Source

    Chimeric HRP conjugated Monoclonal Anti-SN38 Antibody, Mouse IgG1 is recombinantly expressed in HEK293. It consists of mouse variable regions genetically fused to mouse IgG1 constant domains.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    AM685

  • Isotype

    Mouse IgG1, Kappa

  • Host Species

    Mouse

  • Immunogen

    SN38-OVA

  • Conjugate

    HRP conjugated

  • Specificity

    Specifically recognizes the target-SN38.

  • Application

    Application
    Recommended Use / Performance
    ELISA
    1:300-1:3000
  • Purification

    Protein A purified.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Affichage des données

  • Bioactivity-ELISA

     SN38 ELISA

    Immobilized Human TROP-2, Fc Tag (Cat. No. TR2-H5253) at 1 μg/mL, add Sacituzumab Govitecan in the 100% Human Serum and then add HRP conjugated Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-PLM685) at 2 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).

    Protocol
  •  SN38 ELISA

    Immobilized ADC-SN38 at 1 μg/mL (100 μL/well) can bind HRP conjugated Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-PLM685) with a linear range of 2-31.25 ng/mL (Routinely tested).

    Protocol
  •  SN38 ELISA

    Immobilized Human TROP-2, Fc Tag (Cat. No. TR2-H5253) at 1 μg/mL, add Sacituzumab Govitecan in the 0.5% BSA and then add HRP conjugated Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-PLM685) at 2 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).

    Protocol

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FAQ

  • product

    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


Introduction de fond

Dxd (Exatecan ADC derivative) is a potent DNA topoisomerase I inhibitor with an IC50 of 0.31 uM for targeting ADCs (DS-8201A). Dxd was cytotoxic to human tumor cell lines KPL-4, NCI-N87 and SK-BR, 3 and the IC50s of MDA-MB-468 was 1.43 NM-4.07 nM, while lgGADC (Dxd as payload) had no inhibitory effect on HER2 expression in the 4 cell lines. DS8201a (Dxd as payload) had a significant inhibitory effect on HER2-positive KPL-4 and NCI cell lines N87 and SK-BRwith IC50 values of 26.8, 25.4 and 6.7 ng/mL, respectively, but had no inhibitory effect on MDA MB-468 (IC50, > 10000 - mL). HRP-anti-DXD-antibody is a conjugation product of HRP and anti-DXD-antibody. It can be used in PK, PD analysis and ELISA.

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