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PE-Labeled Monoclonal Anti-B2M Antibody, Mouse IgG2b (0.03% Proclin)

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Numéro de produit/spécification
Prix
Qté
B2M-PM614c-25tests
$65.00
B2M-PM614c-200tests
$360.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Source

    PE-Labeled Monoclonal Anti-B2M Antibody, Mouse IgG2b is a mouse monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.

  • Antibody Type

    Recombinant Monoclonal

  • Isotype

    Mouse IgG2b, Kappa

  • Host Species

    Mouse

  • Reactivity

    Human

  • Immunogen

    MOLT-4, a Human acute lymphoblastic leukemia cell line

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Specificity

    Clone BBM.1 detects Beta-2-microglobulin in human.

  • Application

    Flow Cytometry (Evaluation of an epitope within the extracellular domain of human B2M protein).

  • Recommended Dilution

    1:50

  • Isotype Control

    The isotype control is sold separately; please refer to Cat. No. DNP-PM486 for product information.

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months after reconstitution.
    3. 2-8 °C for 12 months after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • Bioactivity-FACS

     B2M FACS

    Flow cytometric analysis of Hela cells staining with PE-Labeled Monoclonal Anti-B2M Antibody, Mouse IgG2b (Cat. No. B2M-PM614c) at 1:50 dilution (2 μL of the stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with negative control cells. PE signal was used to evaluate the binding activity (QC tested).

    Protocol

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Introduction de fond

Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system. B2M consists of two β sheets joined by a disulfide bond, which could form amyloid fibrils in some pathological conditions. Increasing evidence has indicated that B2M gene alterations in tumor cells may lower the efficacy of T-cell based immunotherapies by hampering MHC class I-mediated tumor antigen presentation and avoiding T cell recognition.

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